TAGRA 2024

In this study, laccase enzyme production potentials of Caldibacillus pasinlerensis isolated within the borders of Pasinler district of Erzurum were investigated in the culture medium consisting of medlar and hawthorn seeds. Medlar (Mespilus germanica) and hawthorn (Crataegus sp.) seeds were used as natural substrates. These seeds are in terms of biologically active substances such as lignin, phenolic compounds, flavonoids and tannins and have the potential to stimulate laccase production thanks to their lignocellulose rich osic structures. Laccase enzyme has a wide range of use in biotechnology and applications with its capacity to degrade complex organic molecules such as lignin and phenolic fragments. In experimental study, medlar and hawthorn seeds were ground and divided into 1-2 mm cross-sectional size. The ground seeds were added to the culture medium at different densities (3 g of hawthorn seed and 5 g of medlar seed). In order to investigate the enzyme production potential of the test bacteria, various temperature conditions such as incubation time (72 hours), temperature (55 °C) and pH (9) were optimized. Laccase activity was measured spectrophotometrically using ABTS substrate. The results showed that laccase production was significantly increased in both seed blocks, but the analyses containing the highest enzyme content was obtained from medlar seeds. When evaluated in terms of bioremediation, the utilization of medlar and hawthorn seeds, which have lignocellulosic structure, as agricultural waste offers an economical and sustainable alternative for reducing environmental pollution. The capacity of laccase enzyme to degrade phenolic and aromatic compounds stands out as a promising biocatalytic option especially in the treatment of agricultural and industrial wastewater. This study reveals an important potential for the use of renewable biological resources in industrial enzyme production and environmental remediation processes.