TAGRA 2025

In recent years, considerable attention has been directed toward exploring alternative materials for screws used in jawbone tissue replacement. Nevertheless, issues related to the cytotoxicity and biocompatibility of these materials remain subjects of ongoing debate. In this study, we developed a novel implant for jawbone tissue regeneration by depositing a titanium carbonitride (TiCN) film onto a NiTi shape memory alloy substrate using the Cathodic Arc Physical Vapor Deposition (CAPVD) technique. The investigation focused on evaluating the cellular responses of TiCN-coated NiTi substrates in gingival cells derived from diabetic rats over periods of 1, 15, and 30 days.Initially, the research involved characterizing the NiTi alloy and assessing its distribution within the tissue. Subsequently, several biological parameters—including white blood cell (WBC) components, adenosine triphosphate (ATP) levels, oxidative stress markers, cytochrome c release, and lysosomal membrane integrity—were analyzed across all experimental groups.The findings revealed that diabetic rats implanted with the TiCN-coated NiTi substrate exhibited notably reduced oxidative stress markers, alongside enhanced cell viability, increased intracellular glutathione (GSH) and ATP concentrations, and diminished cytochrome c release and lysosomal membrane damage.Overall, the results suggest that the TiCN-coated NiTi screw demonstrates excellent biocompatibility and minimal cytotoxicity, making it a promising candidate for jawbone tissue replacement in diabetic patients.